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从粪便样本中提取DNA，哪种方案最靠谱

2025-03-16 04:59:15

推荐回答（2个）

回答1：

想从粪便中提取DNA，首先应该先把粪便处理一下的。一般常用10%的赛氏液处理，于粪便中加入赛氏液，3000rpm离心15－20min，取上清液即可用于其他操作，按着protocol提取RNA和DNA都没有问题的。
赛氏液配制：1000ml
1.氯化钠7.65g 2.氯化钾0.2g 3.醋酸钠1.5g
4.磷酸二氢钾0.1.g 5.磷酸二氢钠NaH2PO4H2O 0.05g
6.NaH2PO42H2O 0.05g 7.葡萄糖 1.0g
8.碳酸氢钠 0. 7g 9.维生素C 0.003g
10.无离子水补足1000ml

回答2：

用试剂盒简单快速，免除配化学试剂胡繁琐和毒害！biog粪便DNA提取试剂盒就可以，按说明书操作，安全无毒，粪便预处理后，在15分钟内就可以完成。

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